a级毛片100部免费观看,国产狂喷潮在线观看,精品无码国产自产在线观看水浒传,人妻无码视频一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-1720F9 小鼠畸胎瘤細(xì)胞

F9 小鼠畸胎瘤細(xì)胞

簡要描述:CRL-1720 F9 小鼠畸胎瘤細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種原代細(xì)胞、細(xì)胞系。細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-1720
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:1619

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-1720 F9 小鼠畸胎瘤細(xì)胞


ATCC® Number:  CRL-1720™

Designations:  F9

Depositors:   S Strickland

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Mus musculus (mouse)

Morphology: epithelial

CRL-1720 F9 小鼠畸胎瘤細(xì)胞

Source: Organ: testis

Strain: 129

Disease: embryonal carcinoma; testicular teratoma

Cellular Products: plasminogen activator; laminin; type IV collagen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Roche FuGENE® Transfection Reagents)

Age:  embryo

Comments: F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP).

Differentiating cells synthesize plasminogen activator, laminin and type IV collagen.

cAMP is active only on cells that have been treated with retinoic acid.

The cells maintain three copies of the beta 1 integrin gene.

Tested and found negative for ectromelia virus (mousepox).

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol: NOTE: culture vessels must be coated with 0.1% gelatin prior to use.To do so, cover the surface of the vessel with 0.1% gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperature.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new coated culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1160: Strickland S, et al. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell 21: 347-355, 1980. PubMed: 6250719

1161: Strickland S, Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15: 393-403, 1978. PubMed: 214238

23426: Stephens LE, et al. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression. J. Cell Biol. 123: 1607-1620, 1993. PubMed: 7504677

26151: Berstine EG, et al. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. USA 70: 3899-3903, 1973. PubMed: 4521215

32547: Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649



CRL-1720


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲精品无码永久在线观看| 国产av夜夜欢一区二区三区| 曰韩少妇内射免费播放| 亚洲成a人片在线不卡一二三区| 亚洲精品一区二区| 亚洲男同志网站| 少妇久久久久久人妻无码| 性色av一区二区三区夜夜嗨| 大伊香蕉av最新播放| 久久99精品久久久久久hb无码| 色橹橹欧美在线观看视频高清| 亚洲av无码精品色午夜| 真人直播 免费视频| 久久久久久久岛国免费观看| 国产免费又色又爽又黄软件| 亚洲va无码va在线va天堂| 日本无遮挡吸乳呻吟视频| 亚洲精品欧美综合四区| 人妻丰满熟妇岳av无码区hd| 精品久久久久久久久久久aⅴ| 国产做无码视频在线观看| 久久丫精品国产亚洲av| 国产不卡视频一区二区三区| 黄网站欧美内射| 99国产精品久久久蜜芽| 无码一区二区三区老色鬼| 久久亚洲av永久无码精品| 国产裸体xxxx视频在线播放| 久久精品国产一区二区三区不卡| 人妻系列无码专区久久五月天| 天堂aⅴ无码一区二区三区| 国产精品视频一区二区噜噜| 亚洲国产成人片在线观看| 精品无码久久久久久久久久| 少妇人妻中文字幕hd| 亚洲精品中文字幕乱码三区| 亚洲精品国产精品乱码视色| 国产乱xxⅹxx国语对白| 国产乱人伦偷精品视频下| 水蜜桃av无码| 黄a大片av永久免费|