a级毛片100部免费观看,国产狂喷潮在线观看,精品无码国产自产在线观看水浒传,人妻无码视频一区二区三区

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-11005FL62891細(xì)胞, 人肝細(xì)胞

FL62891細(xì)胞, 人肝細(xì)胞

簡(jiǎn)要描述:FL62891細(xì)胞, 人肝細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-11005
  • 廠(chǎng)商性質(zhì):生產(chǎn)廠(chǎng)家
  • 更新時(shí)間:2024-11-16
  • 訪(fǎng)  問(wèn)  量:1655

產(chǎn)品分類(lèi)

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

FL62891細(xì)胞, 人肝細(xì)胞

ATCC® Number:CRL-11005™    Price:$353.00
Designations:FL 62891

Depositors:ImClone Systems Inc.

Biosafety Level:2 [Cells contain SV-40 viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:fibroblast

Source:Organ: liver
Cell Type: immortalized with SV40 large T antigen


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
FL62891細(xì)胞, 人肝細(xì)胞

Age:fetus

Comments:The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation:ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 32.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 32°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:48 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020



References:70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993
70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994
70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996
70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997
70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998
70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999





















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美日韩亚洲tv不卡久久| 国产性一交一乱一伦一色一情| 久久久久免费看成人影片| 人妻丰满熟妇av无码区免| 国产成人无码av一区二区| √新版天堂资源在线资源| 亚洲av纯肉无码精品动漫| 国产精品欧美福利久久| 久久久久亚洲av成人网人人软件| 免费观看羞羞视频网站| 无遮挡粉嫩小泬久久久久久久| 国产日产精品一区二区三区四区的特点| 成人黄色网址| 丰满少妇人妻无码专区| 欧美午夜精品一区二区三区电影| 狠狠色噜噜狠狠狠狠av不卡| 婷婷亚洲综合五月天小说| 中文字幕人妻中文av不卡专区| 国产精品va在线观看无码不卡| 精品国产一区二区三区四区vr| 日韩欧精品无码视频无删节| 青草内射中出高潮| 国产真实夫妇交换视频| 国产成人免费一区二区三区| 亚洲不卡中文字幕无码| 人人妻人人澡人人爽人人dvd| 久热这里只有精品视频6| 免费无遮挡无码永久在线观看视频| 亚洲日韩国产av无码无码精品| 97一区二区国产好的精华液| 成年无码av片在线| 激情内射人妻1区2区3区| 婷婷久久香蕉五月综合加勒比| 國产一二三内射在线看片| 特黄a级毛片免费视频| 国产日产精品一区二区三区四区的特点| 国产欧美va欧美va香蕉在| 精品久久久久久久中文字幕| 色婷婷五月综合久久| 忘忧草社区www日本高清图片| 爆乳熟妇一区二区三区霸乳|