a级毛片100部免费观看,国产狂喷潮在线观看,精品无码国产自产在线观看水浒传,人妻无码视频一区二区三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2190HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

簡要描述:HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2190
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-15
  • 訪  問  量:1940

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

ATCC® Number:CRL-2190™    Price:$272.00
Designations:HK-2

Depositors:RA Zager

Biosafety Level:2 [Cells Contain Papilloma viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: kidney, cortex
Tissue: proximal tubule
Cell Type: human papillomavirus 16 (HPV-16) transformed


Cellular Products:alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

Receptors:epidermal growth factor (EGF), expressed

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 9

D16S539: 11,12

D5S818: 12

D7S820: 10,11

THO1: 9

TPOX: 8,9

vWA: 17,18



Age:*****

Gender:male

Comments:HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney.

The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes.

The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2.

Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078).

Virus produced by the PA317 cells was used to transduce primary PTCs.

Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones.

The cell line appears to be derived from a single cell based on Southern and FISH analysis.

The E6/E7 genes are present in the HK-2 genome as determined by PCR.

The cells retain a phenotype indicative of well differentiated PTCs.

They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin.

The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase.

HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone.

The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen.

HK-2 cells are anchorage dependent.

The cells will not grow in methylcellulose, soft agar or suspension.

HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
艳妇乳肉豪妇荡乳在线观看| 久久精品国产99国产电影网| 波多野结衣免费一区视频| 久久精品国产清高在天天线| 国产特级毛片aaaaaa视频| 国产精品久久久久久久久久免费看| 大学生久久香蕉国产线看观看| 精品亚洲成a人在线观看| 成在人线av无码免费| a一区二区三区乱码在线 | 欧洲| 亚洲av无码专区青青草原| 丰满爆乳在线播放| 亚洲av无码国产丝袜在线观看| 老少交欧美另类| 4hu44四虎www在线影院麻豆| 欧美性大战久久久久久| 极品少妇小泬50pthepon| 级r片内射在线视频播放| 国产亚洲精品aaaa片app| 国色天香精品一卡2卡3卡4| 欧美成人片在线观看| 国产成人av大片大片在线播放| 藏春阁福利视频| 强开少妇嫩苞又嫩又紧九色| 日本高清视频wwww色| 中文字幕肉感巨大的乳专区| 国产精品岛国久久久久| 国产成人精品无码一区二区老年人| 又粗又粗又黄又硬又深色的| 国产精品久久久久久无码| 国产色欲av一区二区三区| 99久久精品国产一区二区| 日本高清视频wwww色| 在线中文字幕乱码英文字幕正常| 亚洲一区二区三区无码久久| 丰满人妻熟妇乱又伦精品软件| 国产男女无遮挡猛进猛出| 欧美激情一区二区久久久| 人人妻人人添人人爽日韩欧美| 国产sm调教折磨视频| 国产成a人亚洲精品无码樱花|